Mechanism of Allicin for Improvement of Hepatic Fibrosis by Regulating Autophagy

Abstract: Objectives: To explore the effects of allicin on expression mitochondrial autophagyrelated proteins in liver tissues hepatic fibrosis (HF) rats. Materials and Methods: 45 SD rats were randomly divided into Normal, Model, Low, Middle High groups. The HF rat model was replicated intraperitoneal injection carbon tetrachloride ethanol gavage for 4 weeks. Detection alanine aminotransferase (ALT) aspartate (AST) serum; HE Masson staining to observe pathological changes; Evaluating LC 3B protein by Immunofluorescence; Western Blotting qRT-PCR methods used detect PINK1, Parkin, Beclin 1, LC3 gene levels tissues. Results: Compared with Normal group, degree group obvious, volume fraction collagen increased significantly (p<0.001), upregulation serum ALT AST (p<0.001, respectively). Up-regulation mRNA 3II/LC 3I ratio respectively); Allicin treated groups had reduced degree, decreased (p<0.05, respectively), tissue LC3B expressions, down-regulated Conclusion:Allicin could improve fibrosis. mechanism might be related down-regulating 3 interfering level autophagy Keywords: Allicin, HF, Autophagy.